Isolation and the 5'-end nucleotide sequence of Bacillus licheniformis alpha-amylase gene
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence and features of the Bacillus licheniformis gnt operon.
Bacillus licheniformis was able to utilize gluconate as the sole carbon source as efficiently as Bacillus subtilis did. Southern analysis indicated that B. licheniformis likely possesses only one gnt determinant. The nucleotide sequence (6278 bp) of the B. licheniformis DNA containing the gnt operon was determined, revealing the five complete open reading frames (ORF; genes). The putative produ...
متن کاملCloning and nucleotide sequence of the penicillinase antirepressor gene penJ of Bacillus licheniformis.
The penicillinase antirepressor gene, penJ, of Bacillus licheniformis ATCC 9945a was cloned in Escherichia coli by using pMB9 as a vector plasmid. The penicillinase gene, penP, its repressor gene, penI, and penJ were encoded on the cloned 5.2-kilobase HindIII fragment of the recombinant plasmid pTTE71. The penJ open reading frame was composed of 1,803 bases and 601 amino acid residues (molecula...
متن کاملIsolation and Nucleotide Sequence of the Gene
When grown under anaerobic conditions, Neisseria gonorrhoeae, the etiologic agent of the sexually transmitted disease gonorrhea, expresses several novel outer membrane proteins. One of these, Pan 1, has an apparent molecular mass of 54 kDa in electrophoresis and is recognized by serum samples from patients with gonococcal infection. The presence of antibodies to this protein in patient sera sug...
متن کاملKinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase.
The irreversible thermal inactivation of Bacillus licheniformis alpha-amylase was studied. A two-step behaviour in the irreversible denaturation process was found. Our experimental results are consistent only with the two-step model and rule out the two-isoenzyme one. They suggest that the deactivation mechanism involves the existence of a temperature-dependent intermediate form. Therefore the ...
متن کاملMutagenesis of Bacillus Licheniformis through Ethyl Methanesulfonate for Alpha Amylase Production
The present study is concerned with the improvement of Bacillus licheniformis strain GCB30 for alpha amylase production in 250 ml Erlenmeyer flasks. Chemical mutation using ethyl methanesulfonate (EMS 50-300 μ/ml) was undertaken for 10-60 min. Twenty eight isolates were selected on the basis of clear zones of starch hydrolysis. Only one isolate designated as B. licheniformis EMS-200 gave 102.78...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1984
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1984.tb08594.x